So, the principle is ‘thinner is better’, i.e., if we make thin enough specimens so that the single-scattering assumption is plausible, and the TEM research will be much easier. When the times of scattering increase, it is difficult to predict what will happen to the electron and to interpret the images and DPs. If the electron is scattered more than once, it is called ‘plural scattering.’ It is generally safe to assume single scattering occurs, unless the specimen is particularly thick. If the specimen is very thin, this assumption will be reasonable enough. Other limitations to high resolu- tion topographic imaging such as surface contamination, the de-localized nature of the in- elastic scattering event and. Free electrons move in waves, just like light. Instead of light, electrons are used in electron microscopy. \) Two different kinds of electron scattering form (a) a thin specimen and (b) a bulk specimen.Īll scattering in the TEM specimen is often approximated as a single scattering event since it is the simplest process. Limitations of Transmission Electron Microscope (TEM) Generally, the TEMs are very expensive to purchase They are very big to handle. By Mubashir Iqbal On NovemIn Basics of Microbiology What is electron microscope An electron microscope is used to examine objects that are smaller than about 0.2 µm, like viruses or the insides of cells.
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